Avaliação de efeitos tóxicos e comportamentais gerados por resíduos de antibiótico em peixes Danio rerio (Hamilton, 1822)

Abstract

Drugs are used for the treatment of several diseases, thus helping to improve the quality of life of humans and animals. Among these drugs are antibiotics, which are widely used in human and veterinary medicine. However, antibiotics have raised concern, as they are not completely absorbed by organisms, which leads to the generation of waste, which are found in aquatic environments. These residues, in turn, are poorly monitored, which reinforces the importance of toxicological and ecotoxicological analyzes, for a better understanding of the effects caused to organisms and the environment. For this work, the fish Danio rerio was the model chosen due to its agile development, easy maintenance, low cost, reproduction throughout the year and also for being widely used as a model for toxicological experiments and behavioral studies. This study aimed to analyze the toxic and behavioral effects generated by the antibiotic tetracycline hydrochloride in Danio rerio fish. Toxicological tests were performed at different concentrations of the antibiotic, over a period of 48 hours, to obtain the CL50, using the Trimmed Spearman-Karber software, in which we reached a value of 273.86 mg/L, later the behavioral test was performed with the concentration 200 mg/L in a exposure of 96 hours. After exposure, the fish were filmed for 10 minutes and the footage was analyzed using the Tox Trac software. The results of the behavioral analyzes showed that the animals had a decrease in activity levels compared to the control, and also showed signs of dysbiosis, indicating that even at low and non-lethal doses, behavioral effects were observed in the animals. [resumo fornecido pelo autor]