Biomarcador inflamatório e caracterização histológica para distinguir graus graves de discopatia degenerativa lombar

Abstract

Background: Intervertebral disc degeneration is a multifactorial process resulting from cellular, biochemical, and structural changes. This study aimed to elucidate possible biomarkers that contribute to the definition of the Pfirrmann scale, mainly in distinguishing between the most severe degrees of Lumbar Degenerative Discopathy. Methods: The intervertebral disc (IVD) was used in a standardized way to evaluate numerous degenerative characteristics using HE, Safranin O/FCF, Masson's Trichrome, Verhoeff and PAS stains. The expression of Macrophages was analyzed in different degrees of severity of the disease and the extracellular matrix's structure was evaluated by scanning electron microscopy (SEM). Results: The stains allowed us to define the tissue degenerative characteristics of IVD. As it is simpler and less expensive than special stains, the data determined that HE staining can be standardized without compromising fidelity and interpretation. The overexpression of the macrophage showed that it could be an interesting biomarker of the severity of the disease, as it statistically differentiated grades IV and V. The evaluation of elastic fibers by SEM can also be seen as an essential factor to be considered as a marker of degenerative scale. Conclusions: HE staining efficiently determines degenerative processes if applied competently by a qualified professional and could replace special stains. Macrophages could be a future biomarker of disease severity and check the quantity and integrity of elastic fibers by SEM. [resumo fornecido pelo autor]