Nanofibras poliméricas por técnica de fiação por sopro em solução - inovação experimental como protótipo em contexto terapêutico do trauma raquimedular

Abstract

Spinal cord injury (SCI) is still considered a big challenge in terms of therapeutics, since there is a lack of effective results expressed by clinical improvement. The application of polymeric nanofibers produced by blow spinning solution (SBS) can be a rational approach, since it can act directly on a lesion site. In this work, we investigate the potential of PVA nanofibers in aqueous solution produced by SBS as a translational therapeutic prototype for SCI. The nanofibers obtained showed a dimensional homology with an average size of 200?400 nm, high degradation rate and good adhesion to local tissue, and neither in vitro nor ex vivo side effects. In in vitro tests, these nanofibers showed an initial decrease in cell metabolism and dynamics, resulting in lower cell viability rate, mitotic index, and percentage of live cells until 48 h exposition, cooling down the environment. After this time point, all cell types tested, including rat resident spinal cord cells, showed an increase in their metabolism, expressing a possible good microenvironment for regeneration. These results show the potential of PVA-based nanofibers as a translational prototype for SCI therapeutic strategy approach. [resumo fornecido pelo autor]