Determinação de compostos não-alvo por HRMS e quantificação de cafeína por RMN em drogas usadas por motoristas profissionais no Brasil

Abstract

Highways are fundamental to the Brazilian economy, as it is the main route for cargo transportation, where around 75% of all goods are transported. Furthermore, economic pressure pushes these drivers to work long hours due to tight deadlines, leading to exhausting working hours and low pay. With that, there is a significant increase in the use of stimulants by these professionals, among these compounds we can highlight the recurrent use of amphetamines, cocaine and its derivatives. Often these stimulants are purchased in the form of rivets at gas stations. In this way, this work seeks to identify target and non-target active compounds in samples known as "Rebites" used by professional drivers of heavy loads in Brazil through various analytical techniques. For this, seven rivet tablets were analyzed by direct infusion high-resolution mass spectrometer, where it is possible to identify caffeine as the major compound. From this, six independent pills were evaluated by quantitative nuclear magnetic resonance in order to quantify this compound, where around 14% to 30% of caffeine was obtained. Still, the samples were analyzed by liquid chromatography to evaluate the reliability of the Quantitative Nuclear Magnetic Resonance and by Field Emission Electron Guns-Energy dispersive X-ray spectroscopy as a possible method of analysis for the forensic scope. The active compounds present in the tested pills are not detected in current toxicological tests. The results obtained by liquid chromatography require adjustments in the analytical method so that their results are closer to those obtained by the Quantitative Nuclear Magnetic Resonance technique, however the resonance technique can be used in the forensic area as a complement to mass spectrometry, in addition to demonstrating its viability in view of the results obtained. [resumo fornecido pelo autor]