Ação bactericida (in vitro) de gerador de alta frequência sobre culturas bacterianas comumente encontradas em feridas crônicas

Abstract

In the physiotherapy practices, various electrotherapy techniques are used with antiseptic and antibacterial purposes; however, few studies are found about the action of high-frequency generator in the treatment of diseases causing contamination/infection, such as chronic wounds; nevertheless, the high-frequency generator is known for its antiseptic and bactericidal effect by producing ozone at the surface where it is applied. Considering that the high-frequency generator is inexpensive, easy handling and potentiating action of the healing process through their bactericidal action, this thesis aims to evaluate the antimicrobial effect of highfrequency generator on bacteria of the genus Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii. To analyzed the action of highfrequency generator the following tests are realizes: inhibition zone size; counting colony forming units, flow cytometry using the kit Live / Dead® to verify membrane integrity and rhodamine 123 to assess oxidative stress and temperature evaluation. Concluding that the high-frequency generator has bactericidal action, time and frequency behave as dependents, revealing its action is dose-dependent and acts more significantly in Gram-negative bacteria. Its use does not cause local temperature increase, only the glass electrode and the free radical production increases with time of exposure to the high-frequency generator (833Hz), while cell viability decreased.