Síntese do ácido 2-oxo-1,3-dioxolano-4-carboxílico mediada pelo sistema TEMPO?lacase de Trametes versicolor

Abstract

The oxidation of alcohols to obtain higher-value products is a well-established industrial process. In this context, oxidizing glycerol to produce glycerol carbonate is an interesting strategy because it uses an industrial byproduct to create a simple but versatile molecule with applications in processes that produce refined compounds. Glycerol carbonate is a promising product due to its favorable characteristics, such as non-toxicity, biodegradability, and solubility in water. These properties explain why it is becoming more prominent in scientific and technical literature. It is used as an intermediate in applications in its original form and as a substrate for chemical transformations to unctionalize it, which expands its potential use. Given this, this study focuses on optimizing the biocatalytic conversion of glycerol carbonate into 2-oxo-1,3-dioxolane-4-carboxylic acid. This compound can be used to produce isocyanate-free polymers, pharmaceutical products, and amines, among other products. The oxidation of the alcohol functional group of glycerol carbonate to the corresponding carboxylic acid was promoted by a laccase-mediator oxidative system, composed of the laccase enzyme from Trametes versicolor and the redox mediator TEMPO (2,2,6,6 tetramethylpiperidyl-1-oxyl radical). The reaction parameters, including buffer composition and pH, reaction temperature, reaction time, oxygen requirement, reduction in the amount of reagents, and product purification protocols, were evaluated. The proposed biocatalytic system demonstrated high efficiency, converting 99% of glycerol carbonate to 2-oxo-1,3-dioxolane-4 carboxylic acid. Thus, this work proposes a promising biocatalytic route for producing 2-oxo-1,3-dioxolane-4-carboxylic acid, which reinforces the viability of sustainable enzymatic processes for valorizing industrial by-products. [resumo fornecido pelo autor]