Avaliação do tempo de vida cronológico em Saccharomyces cerevisiae em diferentes fontes de carbono associadas com o metabolismo e com os mecanismos de reparação de DNA

Abstract

A diet rich in carbohydrates is one of the few environmental factors capable of interfering in longevity and in an aging of organisms. In this sense, the increased generation and/or accumulation of AGES (Advanced glycation end-products) formed by reactions between monosaccharides glucose and fructose and/or their metabolic intermediates with nucleic acid and amine group of proteins determine the importance of these products for the lifespan. The AGES appear to be associated with a series of diseases related with longevity and aging. In addition, it has been reported an increased in AGEs in diabetes mellitus and neurodegenerative diseases, e.g. Alzheimer´s and Parkinson´s diseases. In this work we search for an association between carbohydrate metabolism and DNA repair mechanisms. The data showed a significant interaction between key enzymes of carbohydrates metabolism with DNA repair mechanisms and indicated that both processes seems to be essential for the maintenance of genomic integrity in yeast. In this work it was also verificated the chronological lifespan (CLS) in several strains of yeast Saccharomyces cerevisiae grown on different carbon sources. The results generated were related with carbohydrate metabolism and DNA repair mechanisms. In addition, CLS was associated with the absence of Tor1 (Target Of Rapamycin), a genetic mechanism that has been associated with aging and longevity. Furthermore, the data generated by the current study allowed to obtain important data about carbohydrate metabolism and its association with longevity and the aging of yeast.