Avaliação de fungos entomopatogênicos como agentes de controle biológico da lagarta-enroladeira-da-macieira Bonagota salubricola (Meyrick) (Lepidoptera, Tortricidae)

Abstract

The apple tree is deprecated by many insects, especially by leaf-roller-apple Bonagota salubricola (Lepidoptera: Tortricidae). Its control in commercial orchards is done almost exclusively with chemical insecticides. Alternatively, this work aimed at the evaluation of the in vitro susceptibility of larvae of the second ínstar of Bonagota salubricola to isolates of Nomuraea, Beauveria and Metarhizium fungus. Each group was treated with suspensions ranged of 2x105 con/mL a 2x109 con/mL, except for the control group, treated with solution Tween-80 0.1%. Also was evaluated the pupal survival, expression of proteases and compatibility with chemical insecticides. One strain of Beauveria and three strain of Metarhizium were pathogenic to B. salubricola, and the levels of virulence increased depending of conidia concentration. The strain E6 of M. anisopliae stood out for marking a control up to 88% with LT50 of 1.66 days, the largest virulence related positively to the increased production enzyme, and this strain was also compatible with tebufenozide. Those results demonstrate the potential of entomopathogenic fungi in control of B. salubricola, principally on the orchards IPM.