A adsorção de tióis monitorados pelo método de voltametria cíclica com uso de eletrodos de ouro

Abstract

In this work, the application of amorphous magnetoelastic alloy coated with gold by magnetron sputtering and electroless methods is demonstrated. The thickness, delamination, and roughness evaluations of the gold coatings show that the working electrode constructed with the amorphous magnetoelastic alloy with gold coating by the electrodeposition method is viable. It can be used for biosensors and bioelectrodes manufacture using the protocols that have thiols and bioconjugates and can be evaluated by electrochemical methods. The electrochemical method of cyclic voltammetry is applicable for the use of gold-plated working electrodes. The simulations demonstrated indicate that it is possible to check the functionality since they operate by diffusion and thus monitor the thiols adsorption. In the voltammetric evaluations of thiol adsorption, it was verified that it is necessary to stir the electrochemical cell during the adsorption to form the self-organized layers in times of up to 180 min. It is possible to reduce the concentration of thiol, but there may be consequences such as increased adsorption time. The work electrodes produced with the gold-plated magnetoelastic amorphous alloy by electrodeposition presented a better performance in the thiol adsorption when compared to the magnetron sputtering coating method. In the production of the gold nanoelectrodes, it was verified that it is possible to improve the signal acquisition with the reduction of the capacitive current, maintaining the characteristics of sigmoidal peaks of macro scale, but using micro and nano scales.