Capacidade de tamponamento salivar associado ao isolamento de microrganismos cariogênicos em biofilme e cárie de pacientes da clínica materno infantil da UCS – estudo piloto

Abstract

The use of saliva in tests of cariogenic activity has shown as an additional advantage for its use in pediatric patients, due to its noninvasive nature and simplicity of sample collection. Characterizing and comparing salivary fluid properties with biofilm and caries of each patient is important to understand the risk of developing carious lesions. In this sense, this pilot study aimed to evaluate the effect of salivary buffer and isolate cariogenic microorganisms present in the biofilm and caries of children from 4 to 10 years old, attended at the Maternal Infant Clinic of the University of Caxias do Brasil Dental School. For this purpose, a sample of 12 voluntary donors were selected, compared with the clinical use data of the patients they were using, such as the number of active lesions, number of teeth restored and presence of biofilm. With saliva specimens, flow and salivary volume tests, buffering capacity and acid production by bacteria present in saliva by the Snyder method were performed. The selected saliva production was evaluated visually by the presence of a pH indicator without glucose culture medium. With biofilm and caries, were performed experiments to isolate and evaluate the presence of cariogenic microorganisms in the samples tested. Gram tests, catalase and determination of colony formation in plates were used. From the Snyder test it was observed that only one sample was negative at all time intervals evaluated. 10 of the 11 samples showed negative results within the first 24 hours, 8 positive after 48 hours and 2 positive after 72 hours. The ceod and CPO-D values were high, 6.4 and 4.5 respectively. It is concluded that there is a relationship between ceo-d / CPO-D and salivary flow and acids produced by bacteria present in saliva, since the smaller the number of caries and restorations, the greater the volume of salivary flow and the higher the salivary flow indices observed for the Snyder test (sic).