Obtenção e caracterização de curativos de látex de borracha natural com adição de hidroxiapatita e nanopartículas de prata para usos biomédicos.

Abstract

In this work, NRL films were enriched with hydroxyapatite and silver nanoparticles in order to obtain dressings with specific properties - such as wettability, roughness, bactericidal activity - to prevent cell adhesion and allow tissue regeneration without contamination by microorganisms. Calcium phosphate and nanoparticulate metal were incorporated into the surface of the polymeric matrix using the magnetron sputtering technique, generating six samples with different parameters. In general, modified NRL films showed hydrophilic character (contact angle between 23° and 42°), a favorable condition for biomedical applications, and with increased roughness. The statistical analysis of the experimental design proved that the process parameter that actually influences the wettability of films is power. The RBS results showed that silver is likely to be in the form of nanoclusters on the surface of the samples. Furthermore, the calcium phosphate stoichiometry was obtained and the Ca/P ratio found was 0.7, indicating that there will be a greater degradation of the ceramic material in the wound bed. MTT cell viability assays revealed that non-cytotoxic effects were observed for almost all sample extracts (viability above 70%) and halo agar analysis showed that the membranes showed bacteriostatic activity against Staphylococcus aureus and E. coli. This work innovates in the addition of calcium phosphate together with latex, as well as in the application of surface engineering to modify this material. It was shown in the in vitro study that enriched NRL films should be considered promising for application in wound healing. [resumo fornecido pelo autor]