Avaliação dos principais metabólicos secundários por espectrometria de massas e atividade hipoglicêmica de Salacia impressifolia Miers A. C. Smith

Abstract

The use of plants in various ailments healing is traditionally known and used for centuries by mankind. Plant cells produce a large variety of chemical compounds between the primary and secondary metabolism. The secondary metabolism products are of special interest, as they are responsible for several biological activities. Ethnobotanic information indicates that more than 800 plants are used as traditional remedies in the treatment of diabetes. Many species of Salacia genus have been described with antidiabetic activity, including Salacia impressifolia. This specie belongs to Celastraceae family, popularly known as miraruíra in Brazil. This work aimed to develop the chemical composition of this specie through stem and stem bark extracts. Chemical analysis of the extracts was carried out by high resolution mass spectrometry (HRMS). In these extracts, 32 compounds were identified, previously described for the same genus. Among them, were identified kotalanol, salacinol and mangiferina, with hypoglycemic action previously described by others authors. For in vivo analysis of hypoglycemic activity, it was performed the hydroalcoholic stem extract in streptozotocin induced diabetics rats. The extract presented similar action to control drug glibenclamide. It was also assessed the lipid profile (triglycerides, total cholesterol and HDL) and liver enzymes AST and ALT, and it was observed an increase in HDL level.