Indução de hiperglicemia in vitro e remediação por extrato natural de LGM3

Abstract

LGM3 is characterized by being a small tree with fruits that have an excellent aroma and among its chemical components, phenolic compounds stand out, including flavonoids and non-flavonoids. The peels are waste by-products and an abundant source of compounds with antioxidant capacity. In this way, they can contribute to the management of several chronic diseases, including Diabetes Mellitus (DM). DM is a metabolic disorder characterized by the constant presence of high concentrations of glucose in the bloodstream, causing numerous pathological effects in the individual, associated with oxidative stress, nitrosative and endothelial dysfunction. The objective of this work was to evaluate the antioxidant activity of LGM3 extract by the DPPH radical scanning method, to quantify total polyphenols by the Folin-Ciocalteau method and to verify their effects on human vascular endothelial cells (EA.hy926) exposed to high concentration of glucose (35mM, HG). Cell viability was measured by the MTT assay and oxidative and nitrosative stress were evaluated, respectively, by the TBARS test and Nitric Oxide production by the Griess technique. The results showed that the LGM3 has high levels of phenolic compounds concentrated, mainly, in the peel and significant antioxidant capacity. The peel extract did not show cytotoxicity at concentrations from 1 to 200µg/mL, with a 50µg/mL dose effective in inhibiting damage to cell morphology and viability caused by HG, in addition to decreasing the concentration of nitric oxide by 39% and lipoperoxidation by 30%. The result will contribute to the development of new therapies supporting the treatment of vascular endothelial dysfunction caused by DM. [resumo fornecido pelo autor]