Virulência de Nomuraea rileyi à Spodoptera frugiperda e perfil protéico do secretoma em presença da cutícula do inseto

Abstract

Viticulture is an activity of great economic importance with emphasis on the sustainability of small property and territorial development associated with tourism related activities. Cultivated plants become vulnerable to pathogens and insect pests and the vine has several species considered as pests that reduce their production and profitability, among them, Spodoptera frugiperda, with causes damage in different parts of the plant. Entomopathogenic fungi can provide an alternative to conventional pesticides for controlling pests, they produce enzymes that degrade the insect exoskeleton, such aschitinases and proteases facilitating the infection. In this work, the insecticide potential of the fungus Nomurea rileyi and S. frugiperda was evaluated, as well as the proteins secreted by N. rileyi in the presence of insect cuticle at different time intervals in one-dimensional gel. The fungus N. rileyi presented virulence against S. frugiperda, determining a CL50 of 2 x 109 con / mL with UCS03 strain demonstrating bionseticida activity. In assessing the protein profile of secretome N. rileyi in the presence of insect cuticle, at different times of cultivation, a highly differentiated profile was verified. The highest concentration of protein was found at day 14 of incubation (0.3507 mg / ml) reducing the amount of protein after this period. In the one-dimensional gel analysis was verified different molecular weights of proteins, in the time interval considered being many less than 75kDa. These proteins with different molecular weights may be involved in fungal metabolism. Thus, these results can contribute to the understanding of the infection process of N. rileyi in S. frugiperda, offering potential for the development of new researches and applications in biotechnological processes.