Avaliação da expressão de biomarcadores em lesões intraepiteliais precursoras do carcinoma escamoso do colo uterino

Abstract

Changes in protein expression resulting from cervical cancer development and dependent on human papillomavirus (HPV) have been indicated in the literature, among them there are proteins directly influenced by HPV oncoproteins, such as transglutaminase 2 (TG2), p53; and proteins with modified expression related to cell cycle control changes, such as p16 INK4a a, ki-67 and bcl-2. This study aimed to analyze expressionais levels of TG2, p16INK4a, p53, ki-67 and bcl-2 in High-grade (HSIL) and low-grade (LSIL) squamous intraepithelial lesion and relate this expression to different viral types of HPV. Tissue samples obtained by biopsy were used directed cervical patients, previously genotyped HPV-DNA. Expression quantification were performed by immunohistochemical technique. It was observed in LSIL the overexpression of TG2 (p<0.05) and decreased expression of p16INK4a (p<0.05) although in HSIL, the proportion was reversed (Pearson value = -0.269). P53 expression was variable in relation to the degree of injury. The expression of ki-67 and bcl-2 levels varied depending beyond the basal layer. p16INK4a presented its expression associated with high-risk types of HPV (p<0.001) and TG2 expression was associated with HPV 11 (p<0.05). No significant associations were found between the viral types and the ki-67, p53 and bcl-2 expression. In conclusion, the expression of the marker is affected by cancerous process and by HPV infection, and expression analysis of these markers may reveal new biomarkers associated with cervical cancer process.